There was a significant 50% decrease in viability following treatment with a 0. This product is sold for in vivo animal research use only and is not intended for any diagnostic use or procedures. A copy of the permit or documentation that a permit is not required must be sent to atcc in advance of shipment. In vivoantitumor activity of su11248, a novel tyrosine kinase inhibitor targeting vascular endothelial growth factor and plateletderived growth. The recommended seeding density for mcf7 nuclight red cells is 1,500 to 2,000 cellscm2. Roswell park memorial institute rpmi 1640 medium has since been found suitable for a variety of mammalian cells, including hela, jurkat, mcf 7, pc12, pbmc, astrocytes, and carcinomas. Atcc stands ready to support our customers needs during the coronavirus pandemic. Volumes used in this protocol are for a 75 cm 2 flask.
Proteomic comparison of mcf7 tumoursphere and monolayer cultures article pdf available in plos one 712. This material may not have been produced or characterized by atcc. Mcf 7 cells derived in separate locations using identical culture methods exhibit different morphologies in 3d. One cryopreserved vial of mcf7 nuclight green cells at a density of 1x106 cellsvial. Besides, i got some statements from atcc product information sheet that most of the clusters remain in suspension until after the 2nd subculture. Why are my mcf 7 breast cancer cells not growing properly. Us environmental protection agency office of pesticide. The effect of commercial phytoestrogen products on mcf7 cells. Cell line description biosafety level use restrictions handling procedure for frozen cells handling procedure for flask cultures subculturing procedure medium renewal complete growth medium cryoprotectant medium references replacement policy specific batch information an agreement. Preliminary screening of the viability of mcf 7 cells using the aqueous extract of commonly used spices 0. Threedimensional 3d cultures are increasing in use because of their ability to represent in vivo human physiology when compared to monolayer twodimensional 2d cultures. Follow product information sheet storage andor thawing instructions.
Mcf 7 is a cell line that was first isolated in 1970 from the breast tissue of a 69year old caucasian woman. To atcc valued customers, atcc stands ready to support our customers needs during the coronavirus pandemic. Below is a list of reagents for the propagation, subcultivation and cryopreservation of mdamb231cells. Using atcc media is the best way to guarantee robust cell growth, ensuring that youll have a supply of cells when you need them.
Every step of the way, a wide range of cell health products. Xenogen mcf 7 lucf5 bioware cell line product sheet page 3 of 4 discovery in the living organism xenogencorporationin vivobiophotonic imagingtechnologies 5. Each vial contains a stable population of mcf7 cells expressing the nuclight red fluorescent protein restricted to the nucleus. The mcf 12a cell line is a nontumorigenic epithelial cell line established from tissue taken at reduction mammoplasty from a nulliparous patient with fibrocystic breast disease that contained focal areas of intraductal hyperplasia.
Hazelwood, mo, usa objective this chapter describes the vitek 2 automated microbiology system and its application in the identification of microorganisms. Each vial contains a stable population of 1,000,000 mcf7 cells expressing the nuclight green fluorescent protein restricted to the nucleus. Mcf 7 cells are a wellcharacterized estrogen receptor er positive control cell line cells are positive for cytoplasmic estrogen receptors and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Below is a list of reagents for the propagation, subcultivation and cryopreservation of mcf 7 cells. Mcf7 performance of eppendorf cell culture consumables tc treated procedure. A natural product provides novel structural specialities that may qualify for. Xenogen mcf7lucf5 bioware cell line product sheet page 2 of 4 discovery in the living organism xenogencorporationin vivobiophotonic imagingtechnologies figure 2. The origin of mcf 7 adr has been a matter of debate since mcf 7 adr cells were redesignated nciadrres in 1998. Mcf 7 human breast carcinoma cells atcc htb 22 are suggested as a negative control. Thus, the real origin of mcf 7 adr cells remains more unclear.
These data demonstrate that mcf 7 lines from different laboratories may have unique biological properties, despite having a similar karyotype mcf 7, mcf 7 s, mcf 7 ko. Effect of different spices on the viability of mcf 7 cells. Nevertheless, some mcf 7 cells become floating again. Example for nucleofection of mcf7 cells with egfp cdna. Positive control cell lines include skbr3 human breast carcinoma cells atcc htb 30, ht29 colon adenocarcinoma atcc htb 38 and a431 human vulval carcinoma cells atcc crl 1555. Customers located in the state of hawaii will need to contact the hawaii department of agriculture to determine if an import permit is required. Atcc was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since. These permits may be required for shipping this product. Essen bioscience warrants that this product performs as described on the product label and in all literature associated. Maintaining healthy cells is the key to experimental success and reproducible research results. Induction of cell cycle arrest and apoptosis in caspase3. One cryopreserved vial of mcf7 nuclight red cells at a density of 1x106 cellsvial.
Hi all, i got some problems in mcf 7 morphology and culturing medium. Many recent studies still describe mcf 7 adr cells as originating from the breast cancer cell line mcf 7. Microbial identification using the biomerieux vitek 2 system david h. This material is cited in a us or other patent and may not be used to infringe the claims. Two days following implantation of placebo or estrogen pellets 17. Mcf7adr cells redesignated nciadrres are not derived. Mcf 7 nuclight red cells reach 80% confluence in a 75cm2 flask after 23 days. Of the two mastectomies she received, the first revealed that the removed tissue was benign. Atcc catalog fy 2020 atcc catalog pdf a hard copy of this catalog may be requested by contacting the information center at 3207624600 or email information center. The mcf7 line retains several characteristics of differentiated mammary epithelium including ability to process estradiol via cytoplasmic estrogen receptors and. Receptor status and subsequent sensitivity of subclones of mcf 7 human breast cancer cells surviving exposure to diethylstilbestrol.
Product description hekplus expression system is a complete cell culture system consisting of suspension cells, xenofree, serumfree medium, transfection reagent and boost reagent that is optimized for transient transfection and protein. Mcf7 human breast cancer cells form differentiated. Mcf 7 tam1 atcc crl3435 homo sapiens mammary gland. Genetic variability in a frozen batch of mcf7 cells invisible in. The fundamental differences in karyotype and biological properties of the mcf 7 atcc, and the previously reported differences in dna restriction fragment polymorphism. Product prices and availability are subject to change. Department of biological sciences, virginia tech, blacksburg, va 24061 abstract the hela cell line, named after the patient henrietta lacks, was one the first human cell lines to be used for tissue culture.
Depending on the wishes of the depositor, atcc may be required to inform the patent depositor of the party to which the material was furnished. Principles the vitek 2 is an automated microbiology system utilizing growthbased technology. Below is a list of reagents for the propagation, subcultivation and cryopreservation of mcf7 cells. Mcf7 cells were nucleofected using the cell line nucleofector tm kit v, program p20 and 2 g of a plasmid encoding the enhanced green fluorescent protein egfp. Flavokawain b inhibited the proliferation of mcf 7 and mdamb231 in vitro. Director andor lead analyst should consult the safety data sheet for specific hazards associated with products. The hela cell line, named after the patient henrietta lacks, was one the first human cell lines to be used for tissue culture. Centrifuge cell suspension at 125 x g for 5 to 10 minutes.
Flavokawain b induced cytotoxicity in two breast cancer. Mcf 7 genomonegx technical data sheet effect of kala peptide on transfection efficiency cell mcf 7 human breast cancer cell line culture condition 1 10 4cellswell100. The fundamental differences in karyotype and biological properties of the mcf7 atcc, and the previously reported differences in dna restriction fragment polymorphism. Procedure and analysis follow the instructions below for the proper use and required quality control activities associated with vitek 2 compact. A natural compound from hydnophytum formicarium induces apoptosis of mcf 7 cells via up. Reagents for expanding, subculturing and cryopreserving. Mcf 7 cells were purchased from the same lot number from atcc and derived in two locations utilizing identical protocols. Mcf7 human breast cancer cells form differentiated microtissues. This cell line can be maintained for years under ideal conditions in vitro.
Rohs compliant 3033 2 e s p e ranza, ran c ho san t a mar g ari t a, cali f or ni a 9268 8. Product information sheet for atcc acs4800k american type culture collection ordering info. Receptor status and subsequent sensitivity of subclones of mcf7 human breast. Mcf10a cells apc catalog number clls1069 storage temperature 196 c liquid nitrogen product description compozr zinc finger nuclease zfn technology is a fast and reliable way to manipulate the genome in a targeted fashion. Us environmental protection agency office of pesticide programs. Cultiloops enable standardised cultures for quality control testing to be prepared quickly, easily and safely.
Mcf7 stands for michigan cancer foundation 7 referring to the institute where in 1973 the cell line was discovered by. A natural compound from hydnophytum formicarium induces. Mh chocolate agar 1 broth bhi broth 35 days 35 2c 5% co 2. The mdamb231 breast cancer cell line was obtained from a patient in 1973 at m. Cell growth and morphology was monitored on day 1, 2 and 3 postseeding. In the decades since its origin, it has become a feature in labs across the world. Mcf 7, mdbk and chang liver cells were maintained in dmem. Shop online for a wide selection of microbiologics escherichia coli atcc 706 for culture purposes and qc.
Despite its longevity, repeated detection of most hela marker chromosomes have lead the scientific community to view hela as a stable cell line and its corresponding findings to be. Biological differences among mcf7 human breast cancer cell. Mcf 12a was derived from adherent cells in the population. Why are my mcf7 breast cancer cells not growing properly. Mcf7 atcc htb22 homo sapiens mammary gland, breast. Mcf 7 cell proliferation assay of estrogenic activity this nomination application includes no confidential information. In addition, all cells in our nuclight catalog have been certified mycoplasma free by atcc and our stable populations have been unambiguously authenticated using ats short tandem repeat str profiling. Mca102a was derived from adherent cells in the population. Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services. Dillenia suffruticosa root dichloromethane extract dcmds has been reported to exhibit strong cytotoxicity towards breast cancer cells. Mtt analysis was performed to analyze the cytotoxic effects of flavokawain b on two breast cancer cell lines, mcf 7 and mdamb231, as well as on the nontransformed mammary epithelial cell line, mcf 10a. This nomination application includes no confidential.
Genetic variability in a frozen batch of mcf7 cells. Biological differences among mcf7 human breast cancer. Nevertheless, some mcf7 cells become floating again. While we are not currently experiencing delays due to this pandemic, we expect that we could see them as the situation evolves.
Bw119262rev5 growth curve of mcf7 redfluc cells figure 1. These data demonstrate that mcf7 lines from different laboratories may have unique biological properties, despite having a similar karyotype mcf7, mcf7 s, mcf7 ko. Mcf 7 cells were seeded in appropriate culture medium rpmi 1640, 10 % fbs. The mcf7 cell line was derived from a 69yearold caucasian female in 1970 and is the most extensively studied and frequently used of all the human breast cancer cell lines. When grown in 3d using scaffoldfree agarose hydrogels, mcf 7 human breast cancer cells selforganize to form directionallyoriented microtissues that contain a luminal space, reminiscent of the in vivo structure of the. Reagents for expansion, subculturing and cryopreservation of mcf 7 cells 5. Cultured cells from the mcf7 breast adenocarcinoma, atcc htb22 cell line were fixed with bd cytofix fixation buffer cat. Mcf 7 human breast cancer cell lines atcc, manassas, va, usa were cultured in roswell park memorial institute rpmi1640 medium sigma, mo, usa supplemented with 10% fetal bovine serum and antibiotics 100 uml penicillin and 100. This line was established from the same tumor material as mcf 10a atcc crl10317 and mcf 10f atcc crl10318. Mcf7 is a cell line that was first isolated in 1970 from the breast tissue of a 69year old caucasian woman. Supplement the above with 10% hyclone fetal bovine serum. Nonetheless, mtt is an extremely robust assay and is metabolised by. The present study was designed to investigate the cell cycle profile, mode of cell death and signalling pathways of dcmdstreated human caspase3 deficient mcf 7 breast cancer cells. Mcf 7 cells derived in another lab were cultured for 5 a, 7 b and 10 c days in 3d.
Rpmi 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. Mcf 7 a common model in phytoestrogen research, the mcf 7 cell line is an epithelial adenocarcinoma cell line derived from human breast tumor. The atcc cell biology collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines from which to choose. Reagents for expansion, subculturing and cryopreservation of mcf7 cells 5. These ready to use, disposable bacteriological loops contain stabilised viable microorganisms. Comparative genomic hybridization of two technical replicates of mcf 7 cells from atcc lot number 59388743 from johns hopkins university at. As our data demonstrate, seemingly similar cells from the same atcc batch, that show the same str geno types for cell line authentication and.
In some cases, media formulations differ slightly from other commercially available equivalents. Mcf7 breast adenocarcinoma national cancer institute. Thank you for your understanding, patience and flexibility as atcc does everything it can to help reduce the impact of. Essen bioscience warrants that this product performs as described on the product label and in all literature. Mcf7 cells human breast adenocarcinoma cell line cell. Nov 30, 2010 mcf 7 adr cells have been widely used as a multidrugresistant breast cancer cell model in cancer research. Thank you for your understanding, patience and flexibility as atcc does everything it can to help reduce the impact of the coronavirus pandemic to our valued customers. My mcf 7 cell line was from atcc, and its recommended medium is minimum essential medium eagle with 2 mm lglutamine and earles bss adjusted to contain 1. Zfns are synthetic proteins engineered to bind dna at a sequencespecific location. Essen bioscience warrants that this product performs as described on the product label and in all literature associated with the sale of said product when used in accordance.